Induction and identification of autotetraploidy in ashwagandha (Withania somnifera Dunal.)

Seed treatment with aqueous colchicine solution was carried out for induction of autotetraploidy in ashwagandha in four accessions (Shilpa, Acc. 17, Bydyanath and MWS-327). Increased stomatal size and decrease in stomata density aided in detection of autotetraploids. Among four ashwagandha accessions tried, Acc. 17 was more responsive for the induction of autotetraploids. Colchicine at 0.5 per cent seed treatment was found to be more effective for eight hour duration. Among three different durations of treatment (3, 6 and 8h), 8h treatment showed better in expression of tetraploid characters.